Opera Medica et Physiologica

Archive

Issue 1 | March 2019

Published ahead of print March 29, 2019; Printed March 30, 2019; OM&P 2019 Volume 5 Issue 1, pages 1-6; doi:10.20388/omp2019.001.0062
Abstract Full Text

Axo-axonal interactions of neuronal cells play an important role in functional development during embryogenesis. Axons of the cells formed on early stages of the brain development provide a template for the growing axons of later axons. However, the mechanisms of the guiding of younger axons by already formed axons are not well understood. In this study, we present a method to study such axo-axonal interactions in vitro using microfluidics methods and culturing neocortical cells. We studied the dynamics of axon growth in microchannels perpendicularly intersecting with other microchannels. This study provides fundamental understanding of the axonal navigation in microfluidic structures, which further facilitate the design of  experimental in vitro model for studying the role of already formed axons in the development of neuronal system.

 


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