Opera Medica et Physiologica

Growing Unidirectional Synaptic Architecture in Dissociated Neuronal Cultures Using Microfluidic Methods

Author Affiliations

Y. Pigareva 1, A. Gladkov 1, 3, V. Kolpakov 1, E. Malishev 2, V. Kazantsev 1, I. Mukhina 1, A. Pimashkin 1

1 Nizhny Novgorod Neuroscience Center, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina ave., Nizhny Novgorod, Russia, 603950

2 Saint-Petersburg Academic University - Nanotechnology Research and Education Centre of the RAS, Saint-Petersburg, Russia

3 Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia

Corresponding author: 

Y. Pigareva (risssum@gmail.com)


In this study we developed microfluidic structure with two neuronal cultures grown in separated chambers and connected by microchannels for axon outgrowth. We estimated bursting activity transfer characteristics between chambers in relation toculture development and determine rate of axonal growth in chambers.