Previously, we reported that the hypomagnetic field obtained by the 100-fold deprivation of the geomagnetic field affected human cognitive processes as estimated in four different cognitive tests. The 40-minute exposure to the hypomagnetic field caused a statistically significant increase both in the task processing time and in the number of errors. The magnetic effect averaged over 40 healthy subjects was about 1.7%. In the present work, the results of a simultaneous study are described, in which the right eye of each subject was video recorded, while the subject performed the tasks. The pupil size increased in the hypomagnetic field. This effect has been calculated by processing the large data set of a few million video frames. The average magnetic effect was about 1.6% (<<0.01, ANOVA, factor of subjects - fixed). Given the heterogeneity, the effect was close to being significant (0.07, ANOVA, factor of subjects - random). The simultaneous recordings of magnetic reactions both for the different cognitive tests and for the eye pupil size were not correlated. These findings provide experimental confirmation of the random nature of the non-specific magnetic biological effects in humans.
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Ovarian cancer remains one of the most common causes of death from gynecological cancer in women world-wide. As is known, the course of ovarian cancer depends on many factors, including genetic and epigenetic disorders. MicroRNAs are currently considered one of the most promising prognostic and diagnostic markers for solid tumors. The purpose of this work was to investigate the DNA methylation level of miR-663a and miR-663b in 25 paired tissue samples from patients with an established diagnosis of ovarian cancer and various histological and clinical characteristics by MS-HRM method. Our results indicate a lower frequency of miR-663a methylation in ovarian tumor tissues (0.09% ± 0.01) compared to histologically normal tissues 0.16% ± 0.01 (p = 0.01). However, an analysis of the miR-663a and miR-663b microRNA gene methylation level in patients with different clinical parameters, including the stage of disease development, the degree of cell differentiation, the occurrence of distant and regional metastases, as well as therapeutic pathomorphosis, not identify statistically significant differences in the methylation levels of these mi-croRNA genes with any of the clinical characteristics, p > 0.05. Thus, our results indicate a potential role of aberrant methylation of the miR-663a microRNA gene in ovarian cancer carcinogenesis. However, additional researches on larger sample sizes are needed to confirm the results obtained.
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| 12_117-126.pdf | 1.86 MB |
Introduction: acute limb ischemia is characterized by a sudden and severe reduction in blood supply to the limb, posing a critical threat to its viability. The incidence of complications associated with ALI remains significantly high. Currently, there is no consensus regarding which preoperative factors influence the incidence of complications in different revascularization methods, nor to what extent they contribute to postoperative outcomes. An analysis of the key predictors of complications will facilitate the development of preventive strategies and establish criteria for selecting the most appropriate treatment approach for ALI. Materials and methods: this study analyzes the outcomes of two treatment methods for ALI classified as Rutherford class II: open surgical intervention (Group I, n = 50) and endovascular procedures (Group II, n = 50). Results and discussion: the composition and impact of preoperative risk factors differed between the two revascularization methods. In the open surgery group, patients with an inflammatory, hypovolemic, or thrombophilic etiology of ALI (11) demonstrated a higher likelihood of an unfavorable postoperative course. Additionally, advanced patient age (7.1), Rutherford ischemia class IIB (0.2), a history of CAD (6.1), DM (9.2), CKD (12.1), and RF (8.5) were identified as significant risk factors for postoperative complications. In the endovascular revascularization group, among perioperative risk factors, the following variables demonstrated significant associations with postoperative complications: CAD (4.9), CKD (12.4), DM (5.28), RF (11), and Rutherford ischemia class IIB (0.2). Conclusion: selecting an appropriate revascularization method based on an individualized risk factor profile for each patient can effectively reduce the incidence of complications in the early postoperative period.
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| 11_106-116.pdf | 1012.94 KB |
Relevance: neurofibromatosis type 1 (NF1) is a hereditary tumor syndrome occurring with a frequency of 1:3164. NF1 is characterized by severe clinical manifestations with multiple cutaneous and subcutaneous tumors, plexiform neurofibromas, skeletal abnormalities and cognitive disorders. The study of the genetic causes of NF1 can become the basis for prenatal diagnosis and the use of new methods of treating the disease. Materials and methods: clinical and epidemiological study of NF1 patients in the Republic of Bashkortostan, sequencing the NF1 gene in their DNA samples as well as whole genome sequencing using the WGS method. To search for the pathogenic variants we found in publications by other authors, we analyzed the Scopus, WoS, ClinVar, PubMed, and SNP databases. Results: the frequency of occurrence of NF1 in the republic is 1:7407. 23 nonsense pathogenic variants in 21 exons of the NF1 gene were identified in 39 NF1 patients from 26 families. Discussion and conclusion: the frequency of development of the main clinical manifestations of NF1 in patients from the republic corresponds to data from around the world, however, plexiform neurofibromas, optic nerve gliomas, short stature and decreased intelligence were detected significantly less frequently. Of the 23 nonsense pathogenic variants we identified, 16 pathogenic variants were previously described by researchers from various countries, and 7 pathogenic variants: NM_001042492.3:c.55G>T (NP_001035957.1:p.Glu19Ter), NM_001042492.3:c.2806A>T (NP_001035957.1:p.Lys936Ter), NM_001042492.3:c.3284T>A ( NP_001035957.1:p.Leu1095Ter), NM_001042492.3:c.5014G>T ( NP_001035957.1:p.Gly1672Ter), NM_001042492.3:c.5242C>T (NP_001035957.1:p.Arg1748Ter), NM_001042492.3:c.7365T>G (NP_001035957.1:p.Tyr2455Ter) and NM_001042492.3:c.7482G>A (NP_001035957.1:p.Trp2494Ter) have been identified for the first time in the word. Patients with nonsense pathogenic variants have significantly higher rates of brain tumors and epilepsy compared to all NF1 patients in the republic.
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| 10_91-105.pdf | 2.79 MB |
This paper presents the possibilities of using the Unity engine to create the research software for cognitive neuroscience using the brain-to-brain synchrony paradigm. Neurofeedback software for neuroscience research in the brain-to-brain synchrony paradigm is represented by software that allows to implement three research protocols based on the EEG signals recording: the “eyes-to-picture” in the double glasses HTC VIVE PRO protocol, the “eyes-to-eyes”/“ face-to-face” protocol and the “eyes-to-screens” protocol. The presented brain-to-brain synchrony software solution for the implementation of different protocols is a digital platform allowing to perform hyperscanning of electrical activity of the cerebral cortex simultaneously in several persons and thus study the functions of higher neural networks of socially determined areas of the cerebral cortex.
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| 09_83-90.pdf | 1.75 MB |
There is currently a significant lack of information on genetic diversity Mycoplasma hominis, associated with urogenital tract infections in the world. Extended survey multilocus sequence typing and phylogenetic analysis of nucleotide se- quences of the genome of Mycoplasma hominis clinical isolates isolated in the Nizhny Novgorod region was conducted using NGS technology. Molecular profiling based on MLST sequence typing of housekeeping genes (ST-type) (gyrB, tuf, ftsY, uvrA, gap) and MVLST virulence genes (VT-type) (p120', vaa, lmp1, lmp3, p60) was defined with using the server https://pubmlst.org. Extended eMLST typing of Russian Mycoplasma hominis isolates was conducted for the first time. 78 new allelic variants of genes uvrA, gyrB, ftsY, tuf, gap, p120′, vaa, lmp1, lmp3, p60 Mycoplasma hominis isolates were deposited in the PubMLST database. Based on phylogenetic analysis, a high degree of genetic diversity of mycoplasma isolates has been established, the first ones are identified and deposited into the database PubMLST new previously undescribed ten sequence (ST) and ten pathotypes (VT) of Mycoplasma hominis Russian isolates.
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| 08_72-82.pdf | 1.38 MB |
Aim: the effect of cryopreservation and molecular hydrogen (Н2) on the sperm quality characteristics, metabolic, oxidative and antioxidant indices of spermatozoa. Sperm diluted with BioXcell diluent, sperm after cryopreservation and sperm after cryopreservation with Н2 were studied as comparison groups. Qualitative indices of spermatozoa, intensity of free-radical processes, activity of antioxidant enzymes and ATP concentration were measured in all groups. Lipoperoxidation was found to be reduced, and antioxidant activity was increased in sperm after cryopreservation with Н2. It was shown that the content of diene, triene conjugates, malonicdialdehyde decreased in spermatozoa and the activity of superoxidedismutase and catalase increased in sperm after cryopreservation with Н2. Reduction of oxidative processes under the action of Н2 was accompanied by an increase in the concentration of ATP in spermatozoa. After thawing, a significant preservation of spermatozoa motility was recorded under H2 action compared to the group without H2. Thus, H2 is effective in its ability to quench reactive oxygen species and thereby protect spermatozoa from the effects of oxidative stress during cryoconservation. These properties of H2 protect spermatozoa at low temperatures and determine the preservation of high functional activity of spermatozoa after thawing.
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| 07_66-71.pdf | 632.34 KB |
Helicobacter pylori (H. pylori) is a bacterium distributed worldwide and common in developing countries. In addition to its gastric manifestation, it has a potential role in the development of non-digestive tract disorders like cardiovascular diseases including dyslipidemia, diabetes, obesity, hypertension, chronic kidney disease, and liver disease. It is unclear whether diabetics are more liable for H. pylori infection or whether H. pylori infection raises the risk of diabetes. This study aims to evaluate the glycemic measures in patients having gastritis with and without H. pylori. A community-based cohort study was carried out on patients diagnosed with gastritis by gastroscopy examination. Our sample was divided into H. P-positive group (150 female & 155 male) and H. P-negative group (175 female & 68 male). Diagnosis of Helicobacter pylori infection was done by 13C urea breath test. A control group (60 female and 60 male) that had neither gastritis nor H.P infection were included in this study. Laboratory investigations were performed after a 10 h fast, including fasting blood glucose (mg/dl), fasting serum insulin (IU/ml), HbA1c and 2h postprandial plasma glucose. HOMA-IR index was used to study the relation between Helicobacter pylori and insulin resistance. Helicobacter pylori infection was significantly associated (p < 0.05) with glycemic measures in patients with gastritis. In gastritis with and without Helicobacter pylori, glycemic measures were significantly (p < 0.05) increased in females. It can be concluded that by causing insulin resistance, H. pylori contributes to diabetes and may be magnified to promote long-term diabetes.
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| 06_58-65.pdf | 837.43 KB |
Hexavalent chromium Cr (VI) causes reactive stress and inflammation in the heart; hence this study examines how oxidative stress from Cr (VI) affects the circulatory system and the inflammatory and oxidative processes that generate it. Fifty mice were split into five groups. One group was a control, while four received oral Cr (VI) (5 mg, 10 mg, 20 mg, and 50 mg) daily for 30 days. At the conclusion of the study, blood concentrations of ATP, troponin I, and CK-MB were assessed for cardiac injury. To assess oxidative stress, glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) were examined. Inflammatory biomarkers, including tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), were identified in a composite of cardiac tissue. Significant cardiac injury and reactive stress were evidenced by the substantial increases in CK-MB, troponin I, and MDA levels in relation to dosage. Antioxidant markers like CAT, SOD, and GSH went down a lot, which means the body's antioxidant defenses were not as strong as they used to be. Increased TNF-α and IL-1β levels, particularly with larger Cr (VI) doses, indicated an inflammatory response. Research demonstrates that Cr (VI) causes oxidative stress and inflammation in the heart, which worsens with greater doses. This study shows how important it's to find more safety drugs that can protect the heart from the damage that Cr (VI) does.
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| 05_48-57.pdf | 642.86 KB |
The Epstein-Barr virus (EBV) is an oncogenic virus that persists in a latent state within B-lymphocyte cells. This virus has been associated with numerous forms of haematologic malignancies, including leukaemia. The focus has been on the association between single nucleotide polymorphisms (SNPs), such as those in Interleukin-10, and leukaemia patients. The enzyme-linked immunosorbent assay (ELISA) was employed to identify Epstein-Barr virus EBNA-1-IgG, whereas the Sanger sequencing approach was utilised to detect Interleukin-10 SNPs. This study aimed to ascertain the presence of Epstein-Barr virus EBNA-1-IgG in patients with acute lymphocytic leukaemia and to investigate Interleukin-10 single nucleotide polymorphisms (SNPs) -819T˃C (rs1800871) and -592A˃C (rs1800872) in patients with acute lymphoblastic leukaemia. The statistical examination of the immunological assay indicated no significant difference between the proportion of patients positive for EBV EBNA-1-IgG and the positive controls. The statistical analysis reveals a substantial disparity in the proportion of CC genotype carriers at IL10 -819T˃C (rs1800871) between patients and controls within the molecular study framework. A notable difference was seen in the prevalence of TC genotype carriers at IL10 -819T˃C (rs1800871) between patients and controls. A significant difference was observed between healthy controls and patients with AC, AA, and CC genotype carriers at IL10 -592A>C (rs1800872). This study illustrates the notable prevalence of Epstein-Barr virus (EBV) in individuals with acute lymphoblastic leukaemia. This study demonstrates the association between acute lymphoblastic leukaemia and the CC and TC genotypes at IL10-819T˃C (rs1800871), along with the AC, CC, and AA genotypes at IL10 -592A˃C (rs1800872).
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| 04_38-47.pdf | 640.48 KB |
