Methacrylic resins (MAR) are highly sought-after materials for contemporary additive manufacturing of products, exhibiting a broad spectrum of applications. In order to impart new biological properties to the products printed using MSLA-technology, metal titanium nanoparticles (0.001-0.1 w.%) were added to the MAR composition. Samples of composite materials (MAR/Ti-NPs) were printed from the obtained modified resins. The present study demonstrated the ability of the obtained composite materials to enhance the formation of hydroxyl radicals and hydrogen peroxide molecules, to cause oxidative damage to DNA and protein molecules, and to significantly inhibit the growth of E. coli bacterial cultures and cause the death of bacterial cells during prolonged contact with the investigated material samples. The investigations conducted to assess the impact of the obtained composite materials on the proliferation and maturation of HSF cells have indicated an elevated degree of cytocompatibility.
| Attachment | Size |
|---|---|
 05_58-67.pdf | 714.84 KB |
This study investigated the production, purification, and vitro cytotoxic potential of L-glutaminase derived from Bacillus subtilis. Twenty-five bacterial isolates were recovered from diverse soil samples. Identification of B. subtilis was confirmed using standard biochemical assays, differential media, and the VITEK 2 automated system. Screening for L-glutaminase production identified B. subtilis isolate B7 as exhibiting the highest activity, with a crude enzyme-specific activity of 15.8 U/mg. Optimization of enzyme production parameters revealed glucose and yeast extract as optimal carbon and nitrogen sources, respectively, under controlled conditions of 40 °C and pH 6. L-glutaminase was purified using a four-step protocol: ammonium sulfate precipitation, ion exchange chromatography, gel filtration chromatography, and salt precipitation. Gel filtration chromatography resulted in the highest specific activity (754.6 U/mg), representing a 15.2-fold purification and a 21.1% yield relative to the crude extract. The in vitro cytotoxic effects of crude and purified L-glutaminase were evaluated using the MTT assay against the A549 (human lung adenocarcinoma) and WRL 68 (normal human liver) cell lines. Paclitaxel, a known chemotherapeutic agent, was a positive control for cytotoxicity against A549 cells. Treatment of A549 cells with purified L-glutaminase induced a dose-dependent reduction in cell viability, with a concentration of 400 µg/ml achieving maximal inhibition. These findings suggest that purified L-glutaminase from B. subtilis B7 demonstrates significant in vitro cytotoxic activity against A549 lung cancer cells and merits further investigation as a potential therapeutic candidate.
| Attachment | Size |
|---|---|
| 04_43-57.pdf | 1.09 MB |
Pollutants, especially hydrocarbon oil pollutants, are among the most important problems spread worldwide due to the environmental and health problems to all forms of life, especially humans. Therefore, it is important today to find environmentally friendly and low-cost treatment methods to decrease the high risk of pollutants. In this study, the single-celled green algae including Chlorococcum humicola was used to treat different concentrations of anthracene. The results of the High-Performance Liquid Chromatography (HPLC) technique revealed that the alga used had high treatment efficiency, as naphthalene disappeared at concentrations of 100 and 200 ppm after day 12 of treatment. In addition, the results also showed the possibility of using the algae as a carbon source for anthracene.
| Attachment | Size |
|---|---|
| 03_27-42.pdf | 1.11 MB |
Redox dynamics in mononuclear cells during phagocytosis and post-exposure to UV radiation was evaluated in vitro. Mononuclear cells were isolated from rat blood using density gradient centrifugation. Phagocytosis was activated via latex beads, and redox processes were monitored at 1, 30, and 60 minutes in control samples and after UV exposure at doses of 1, 5, and 15 J/m2. Cell viability post-phagocytosis was assessed using propidium iodide staining and cytofluorimetry. Redox processes in phagocytes were evaluated by measuring fluorescence of tryptophan, Schiff bases and glycated proteins, alongside absorption spectra/florescence levels of metabolic coenzymes FAD, NAD(P) and NAD(P)H and their ratios. In controls, cell viability decreased by 15.2% after 60 minutes of phagocytosis, while tryptophan, NAD(P), and NAD(P)H levels remained stable. Concentrations of Schiff bases and glycated proteins doubled. UV radiation significantly reduced cell viability in a dose-dependent manner. At 15 J/m2, 80.99% of cells were nonviable after 60 minutes of phagocytosis. This dose also caused a 21.42-fold decrease in tryptophan fluorescence, a 3.8-fold reduction in Schiff bases/ glycated proteins, and declined metabolic coenzyme levels. Ratios of NAD(P)H/FAD and NAD(P)H/NAD(P) decreased post-UV exposure, indicating oxidative dominance and NAD(P)H deficiency. However, despite UV-induced cytotoxicity, all experimental series, regardless of the dose of UV discharge, maintained control trends: non-enzymatic glycation products (Schiff bases, glycated proteins) increased, and the NAD(P)H/FAD ratio rose due to reduced coenzyme accumulation during phagocytosis. This suggests that phagocytic redox pathways persist under UV stress, albeit at diminished intensity.
| Attachment | Size |
|---|---|
| 02_13-26.pdf | 1.12 MB |
Background: Saliva is an important body fluid that aids in digestion and contains a repertoire of important biomarkers. This research aimed to investigate the role of sodium bicarbonate toothpaste in regulating salivary indexes. Materials and Methods: The study involved taking saliva samples from healthy and fasting adult, male volunteers, aged between 20 to 45 years old. The samples were taken randomly and stratified, from 50 smokers and 50 non-smokers. The levels of metalloproteinase-8, interleukin-1β, pH, salivary flow rate, and α-amylase were measured, and the effects of sodium bicarbonate toothpaste application were investigated. Results: The levels of metalloproteinase-8 and interleukin-1β in smokers were significantly elevated, with more acidic saliva but reduced salivary flow rate and α-amylase. With the application of sodium bicarbonate, there were significant reductions in metalloproteinase-8 and interleukin-1β, with substantial improvement in pH, salivary flow rate, and α-amylase in both smokers and non-smokers. Conclusion: This study confirmed the negative effects of smoking. The use of sodium bicarbonate toothpaste enhanced oral health by reducing the pro-inflammatory biomarkers while improving the pH, salivary flow rate, and α-amylase.
| Attachment | Size |
|---|---|
| 01_5-12.pdf | 702.27 KB |
Previously, it was assumed that somatic cells in male and female individuals had the same molecular pathways of ontogenesis. However, it has been shown that the course of various pathologies is associated with, among other things, genetic sexual dimorphism. It is known that glial cells are associated with the pathogenesis of various diseases, while the effect of sex chromosomes in glial cells has not been studied to date
| Attachment | Size |
|---|---|
| 15_155-162.pdf | 642.82 KB |
Microarrays are one of the modern technologies for analyzing biological polymers (DNA, RNA, antibodies, etc.). The main advantage of microarrays is the ability to simultaneously analyze a large number of different molecules in one sample. Microarrays are actively used to develop diagnostic tools, including in the analysis of community-acquired pneumonia pathogens. The relevance of studying community-acquired pneumonia is determined by the consistently high incidence, diversity of pathogens and their high genetic variability, as well as the recent spread of a new coronavirus infection. This review describes microarray technologies used to solve fundamental and clinical problems in the study of pathogens of community-acquired pneumonia and some other respiratory infections.
| Attachment | Size |
|---|---|
| 14_136-154.pdf | 1.77 MB |
Previously, we reported that the hypomagnetic field obtained by the 100-fold deprivation of the geomagnetic field affected human cognitive processes as estimated in four different cognitive tests. The 40-minute exposure to the hypomagnetic field caused a statistically significant increase both in the task processing time and in the number of errors. The magnetic effect averaged over 40 healthy subjects was about 1.7%. In the present work, the results of a simultaneous study are described, in which the right eye of each subject was video recorded, while the subject performed the tasks. The pupil size increased in the hypomagnetic field. This effect has been calculated by processing the large data set of a few million video frames. The average magnetic effect was about 1.6% (<<0.01, ANOVA, factor of subjects - fixed). Given the heterogeneity, the effect was close to being significant (0.07, ANOVA, factor of subjects - random). The simultaneous recordings of magnetic reactions both for the different cognitive tests and for the eye pupil size were not correlated. These findings provide experimental confirmation of the random nature of the non-specific magnetic biological effects in humans.
| Attachment | Size |
|---|---|
| 13_127-135.pdf | 638.23 KB |
Ovarian cancer remains one of the most common causes of death from gynecological cancer in women world-wide. As is known, the course of ovarian cancer depends on many factors, including genetic and epigenetic disorders. MicroRNAs are currently considered one of the most promising prognostic and diagnostic markers for solid tumors. The purpose of this work was to investigate the DNA methylation level of miR-663a and miR-663b in 25 paired tissue samples from patients with an established diagnosis of ovarian cancer and various histological and clinical characteristics by MS-HRM method. Our results indicate a lower frequency of miR-663a methylation in ovarian tumor tissues (0.09% ± 0.01) compared to histologically normal tissues 0.16% ± 0.01 (p = 0.01). However, an analysis of the miR-663a and miR-663b microRNA gene methylation level in patients with different clinical parameters, including the stage of disease development, the degree of cell differentiation, the occurrence of distant and regional metastases, as well as therapeutic pathomorphosis, not identify statistically significant differences in the methylation levels of these mi-croRNA genes with any of the clinical characteristics, p > 0.05. Thus, our results indicate a potential role of aberrant methylation of the miR-663a microRNA gene in ovarian cancer carcinogenesis. However, additional researches on larger sample sizes are needed to confirm the results obtained.
| Attachment | Size |
|---|---|
| 12_117-126.pdf | 1.86 MB |
Introduction: acute limb ischemia is characterized by a sudden and severe reduction in blood supply to the limb, posing a critical threat to its viability. The incidence of complications associated with ALI remains significantly high. Currently, there is no consensus regarding which preoperative factors influence the incidence of complications in different revascularization methods, nor to what extent they contribute to postoperative outcomes. An analysis of the key predictors of complications will facilitate the development of preventive strategies and establish criteria for selecting the most appropriate treatment approach for ALI. Materials and methods: this study analyzes the outcomes of two treatment methods for ALI classified as Rutherford class II: open surgical intervention (Group I, n = 50) and endovascular procedures (Group II, n = 50). Results and discussion: the composition and impact of preoperative risk factors differed between the two revascularization methods. In the open surgery group, patients with an inflammatory, hypovolemic, or thrombophilic etiology of ALI (11) demonstrated a higher likelihood of an unfavorable postoperative course. Additionally, advanced patient age (7.1), Rutherford ischemia class IIB (0.2), a history of CAD (6.1), DM (9.2), CKD (12.1), and RF (8.5) were identified as significant risk factors for postoperative complications. In the endovascular revascularization group, among perioperative risk factors, the following variables demonstrated significant associations with postoperative complications: CAD (4.9), CKD (12.4), DM (5.28), RF (11), and Rutherford ischemia class IIB (0.2). Conclusion: selecting an appropriate revascularization method based on an individualized risk factor profile for each patient can effectively reduce the incidence of complications in the early postoperative period.
| Attachment | Size |
|---|---|
| 11_106-116.pdf | 1012.94 KB |
