Opera Medica et Physiologica

The Effect of Ionizing Radiation in Combination with Chemotherapy Drug Doxorubicin on A431 Human Epidermoid Carcinoma Cell Treatment


Radiotherapy is one of the most effective and most commonly used methods of cancer treatment. However, as a result of irradiation, there are side effects that occur as a result of ionizing radiation on healthy tissues. The use of a combined approach with the use of low doses of radiation and antitumor drugs that have a radiosensitizing effect may be one of the ways to reduce side effects and overcome the resistance of malignant cells. This study was undertaken to evaluate the combined effects of radiotherapy and the chemotherapeutic agent Doxorubicin on A431 human epidermoid carcinoma cell line. The cells were incubated with the antitumor antibiotic Doxorubicin and then exposed to high-energy electron ionizing radiation. The cell viability was examined using the MTT assay. The results showed that Doxorubicin acts as a radiosensitizer. Moreover, the combined effect of Doxorubicin and high-energy ionizing radiation of electrons is additive. According to the obtained results, combination therapy used in the treatment of oncological diseases can significantly reduce the radiation dose and minimize the side effects that occur during high doses of irradiation.