Opera Medica et Physiologica

Archive

Supplement S1 | December 2015

Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 21-22; doi:10.20388/omp2015.00s1.001
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The medial preoptic nucleus is critically involved in the social type of behavior regulation, such as parental behaviour, social recognition, sexual behaviour, ect.. A big amount of studies are focused on the role of glutamate, GABA, serotonin, and dopamine systems of medial preoptic area (mPOA) in the social types of behaviour regulation. However, the role of glycinergic system in this nucleus has not been investigated.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 20-21; doi:10.20388/omp2015.00s1.002
Abstract Full Text

The aim of the investigation was to assess antihypoxic and neuroprotective properties of the glial cell line-derived neurotrophic factor (GDNF) in hypoxia models in vitro and in vivo. In vitro experiments were carried out on primary hippocampal cultures. Hypoxia modeling was performed on day 14 of culture development in vitro (DIV) by replacing the normoxic cultural medium with a medium containing low oxygen for 10 minutes. Registration of extracellular action potentials was conducted by MEA systems (Multichannel Systems, Germany) application. Study the effect of GDNF on synaptic plasticity was performed using SmartFlare RNA Detection Probes (Merck Millipore, France) and fluorescent microscopy. In vivo experiments were carried out on C57BL/6j male mice. For acute hypobaric hypoxia a vacuum flow-through chamber was used at the ambient temperature of 20–22°C. We have investigated the resistance of animals to hypoxia and their spatial memory retention in Morris water maze test 24 hours after hypoxia. In vitro and in vivo data demonstrated that GDNF has strong antihypoxic and neuroprotective properties. Preventive GDNF application before hypoxia contributed to the animal survival and spatial memory retention as well as the maintenance of cells viability in primary hippocampal cultures. 


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 19; doi:10.20388/omp2015.00s1.003
Abstract Full Text

To identified genetic cause of congenital hypoplasia cerebellum in two families with different syndromes high- throughput sequencing analysis was performed. X-linked non-progressive ataxia in first family from Mongolian ancestry was caused by genetic defects in ABCB7 gene and modifying by ATP7A gene.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 18; doi:10.20388/omp2015.00s1.004
Abstract Full Text

Today many fundamental questions in Neuroscience can be addressed with Microfluidics methods which provide unique approaches for cell patterning and control neural branch outgrowth. Such methods can be used to gwoe separate subpopulations of dissociated neurons connected with uni-directional connectivity. Combined with microelectrode arrays such approach can be used to simulate and study neurogenesis, learning and information coding in neural networks.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 16-18; doi:10.20388/omp2015.00s1.005
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In this study we developed microfluidic structure with two neuronal cultures grown in separated chambers and connected by microchannels for axon outgrowth. We estimated bursting activity transfer characteristics between chambers in relation toculture development and determine rate of axonal growth in chambers.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 15-16; doi:10.20388/omp2015.00s1.006
Abstract Full Text

The aim of the investigation was to study a role of cannabinoid receptors type 1 (СВ1) and type 2 (СВ2) in implementation of antihypoxic and neuroprotective effects of N-ADA in hypoxia model in vitro. The experiments were carried out on primary hippocampal cultures. N-ADA effect on the spontaneous bioelectrical and calcium network activity in dissociated hippocampal cultures in normal and hypoxic conditions as well as the role of CB1 and CB2 in the implementation of these effects were investigated. Registration of extracellular action potentials was conducted by MEA systems (Multichannel Systems, Germany) application. For the detection of patterns of spontaneous calcium oscillations we used fluorescent calcium dye Oregon Green 488 BAPTA-1 AM (Invitrogen) and a confocal laser scanning microscope (Zeiss LSM510, Germany). Study the expression of mRNA CB1 receptors was performed using SmartFlare RNA Detection Probes (Merck Millipore, France) and fluorescent microscopy. Our data demonstrated that N-ADA has strong antihypoxic and neuroprotective properties associated with activation of cannabinoid receptors type 1. 


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 14-15; doi:10.20388/omp2015.00s1.007
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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 12-14; doi:10.20388/omp2015.00s1.008
Abstract Full Text

In this work we report two successful cases of developing neural networks of spiking neurons for controlling mobile robots. In the first case we use a toy robot, a crocodile, driven by a neural network in-silico. We show that this so-called neuroanimat is capable of detecting internal events of synchronization of network responses to stimuli. In the second example we employ a spiking neural network for building a human-robot interface. Using a bracelet with eight electromyographic sensors we have shown that the interface can faithfully detect myographic signals, classify them according to hand gestures, and send the corresponding commands to the robot. Although the two applications belong to different areas of Neuroscience, they are based on a common approach of neural computations. We note that in both cases besides neural networks there are no additional external algorithms for the decision-making. 


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 10-11; doi:10.20388/omp2015.00s1.009
Abstract Full Text

The study was designed to investigate the effect of verapamil on the speed of propagation of electrical excitation in the myocardium. As a result, it was found that verapamil reduces heart rate, the speed of excitation in the myocardium remains unchanged. The results obtained allow us to estimate the effect of verapamil on the condition of cardiac tissue, based on the change in its conductivity during treatment with the drug.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 10; doi:10.20388/omp2015.00s1.0010
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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 9-10; doi:10.20388/omp2015.00s1.0011
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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 8-9; doi:10.20388/omp2015.00s1.0012
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This article may be regarded as a quick reference to laboratory staff who are wishing to develop their own microscopy system for self-service and modernization of the system and in order to save the lab budget.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 7-8; doi:10.20388/omp2015.00s1.0013
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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 7; doi:10.20388/omp2015.00s1.0014
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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 5-7; doi:10.20388/omp2015.00s1.0015
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Single episode of generalized tonic-clonic seizures induced by pentylenetetrazole led to slowly developing memory impairments in rats, accompanied by elimination of excessive newly generated young cells which were born in the hippocampus soon after the seizures and transient activation of microglial cells in this neurogenic niche.


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Published ahead of print December 01, 2015; Printed December 01, 2015; OM&P 2015 Volume 1 Supplement S1, pages 5; doi:10.20388/omp2015.00s1.0016
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