The Nature of Changes in mRNA Expression and the Relative Content of Selenoproteins in a Mouse Model with TAA-Induced Fibrosis and Hepatocellular Carcinoma (HCC)

This work is devoted to the study of mRNA expression patterns and the relative content of selenoproteins in mice with TAA-induced liver fibrosis and TAA-induced HCC. The main objective of this study is to evaluate the activation or suppression of selenoprotein synthesis during HCC progression and directly in the tumor in one TAA-treated mouse model, which is a pilot study and allows us to closely approximate the situation observed during HCC progression in vivo. It was found that as HCC progresses, there is an increase in the mRNA expression of thioredoxin reductases TXNRD1 and TXNRD2, deiodinase DIO3, glutathione peroxidases GPX1, GPX2, GPX4, and an inverse correlation in the expression of mRNA was characteristic of GPX3. In addition, the mRNA expression of endoplasmic reticulum resident selenoproteins: SELENOM, SELENON, SELENOT and SELENOS changed significantly. Also, in tumor liver samples and directly in the tumor itself, an increase in the expression of the selenoprotein SELENOP was recorded. The information obtained from the results of this work will significantly complement the existing data on the role of mammalian selenoproteins in various liver pathologies and in oncogenesis in general.