Effect of Puncture Photobiomodulation on Pain Stress: a Placebo-Controlled Experimental Study in Rats

The aim: determination of correlation dependence of adaptogenic defense reactions in rats in the acute phase of pain stress on laser-puncture exposure to low-intensity IR radiation. Material and methods. Four groups of rats were used in the work: "experimental", which received after the injury a 10-day course of PPBM, and three comparison groups ("intact", "no exposure" and "placebo"). The intensity of lipid peroxidation, specific activity of antioxidant enzymes, protein concentration, malonic dialdehyde content in blood plasma and erythrocytes were determined by spectrophotometric method. Microcirculation indices were evaluated according to the previous series of our studies. Emotional and behavioral reactions were studied in the "open field" test. Results. Normalization of metabolic, microcirculatory and behavioral indices was registered in the experimental group. The correlation coefficients between the microcirculation index and the data of behavioral activity under pain stress were significantly less than the correlation coefficients between oxidative stress and cognitive functions, which confirms the determining role of oxidative metabolism disturbance in the formation of cognitive disorders. Conclusion: the registered adaptation and stress-limiting effect of PPBM in conditions of experimental pain stress allows us to recommend the technology of laser acupuncture for use in complex rehabilitation of patients with pain syndrome.