The aim of the investigation was to study a role of cannabinoid receptors type 1 (СВ1) and type 2 (СВ2) in implementation of antihypoxic and neuroprotective effects of N-ADA in hypoxia model in vitro. The experiments were carried out on primary hippocampal cultures. N-ADA effect on the spontaneous bioelectrical and calcium network activity in dissociated hippocampal cultures in normal and hypoxic conditions as well as the role of CB1 and CB2 in the implementation of these effects were investigated. Registration of extracellular action potentials was conducted by MEA systems (Multichannel Systems, Germany) application. For the detection of patterns of spontaneous calcium oscillations we used fluorescent calcium dye Oregon Green 488 BAPTA-1 AM (Invitrogen) and a confocal laser scanning microscope (Zeiss LSM510, Germany). Study the expression of mRNA CB1 receptors was performed using SmartFlare RNA Detection Probes (Merck Millipore, France) and fluorescent microscopy. Our data demonstrated that N-ADA has strong antihypoxic and neuroprotective properties associated with activation of cannabinoid receptors type 1.
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| 10.20388omp2015.00s1.007.pdf | 499.92 KB |
In this work we report two successful cases of developing neural networks of spiking neurons for controlling mobile robots. In the first case we use a toy robot, a crocodile, driven by a neural network in-silico. We show that this so-called neuroanimat is capable of detecting internal events of synchronization of network responses to stimuli. In the second example we employ a spiking neural network for building a human-robot interface. Using a bracelet with eight electromyographic sensors we have shown that the interface can faithfully detect myographic signals, classify them according to hand gestures, and send the corresponding commands to the robot. Although the two applications belong to different areas of Neuroscience, they are based on a common approach of neural computations. We note that in both cases besides neural networks there are no additional external algorithms for the decision-making.
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| 10.20388omp2015.00s1.008.pdf | 563.34 KB |
The study was designed to investigate the effect of verapamil on the speed of propagation of electrical excitation in the myocardium. As a result, it was found that verapamil reduces heart rate, the speed of excitation in the myocardium remains unchanged. The results obtained allow us to estimate the effect of verapamil on the condition of cardiac tissue, based on the change in its conductivity during treatment with the drug.
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| 10.20388omp2015.00s1.009.pdf | 247.44 KB |
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| 10.20388omp2015.00s1.0010.pdf | 197.16 KB |
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| 10.20388omp2015.00s1.0011.pdf | 351.06 KB |
This article may be regarded as a quick reference to laboratory staff who are wishing to develop their own microscopy system for self-service and modernization of the system and in order to save the lab budget.
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| 10.20388omp2015.00s1.0012.pdf | 336.63 KB |
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| 10.20388omp2015.00s1.0013.pdf | 303.42 KB |
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| 10.20388omp2015.00s1.0014.pdf | 230.06 KB |
Single episode of generalized tonic-clonic seizures induced by pentylenetetrazole led to slowly developing memory impairments in rats, accompanied by elimination of excessive newly generated young cells which were born in the hippocampus soon after the seizures and transient activation of microglial cells in this neurogenic niche.
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| 10.20388omp2015.00s1.0015.pdf | 510.03 KB |
