Cryopreservation significantly impacts sperm integrity, reducing their fertility potential. These processes are largely dependent on increased oxidative stress. Low-level laser irradiation (LLLI) can increase energy delivery to cells and reduce reactive oxygen species (ROS). Our goal was to analyze the effects of LLLI on metabolic, oxidative and antioxidant parameters in cryopreserved bovine spermatozoa and the possible mechanisms of its action. We analyzed 50 samples of bull semen, dividing them into two groups: a control group without laser irradiation and a group exposed to laser irradiation. We also analyzed the effects of LLLI in combination with the adenylatecyclase stimulator - forskolin, the Ca2+ channel blocker - verapamil and the protein kinase C inhibitor - staurosporine. Irradiation was performed in petri dishes of 30 mm containing 3 ml of sperm at a wavelength of 650 nm. Cryopreservation was found to increase lipid peroxidation and decrease metabolism which was accompanied by a decrease in sperm motility and viability of sperm. ATP concentrations was increased while sperm motility and MDA concentrations were decreased during cryopreservation with LLLI. The effects of LLLI varied depending on the added substances. A combination of LLLI and forskolin demonstrated increased motility and metabolic activity compared to LLLI alone. The effects of LLLI were significantly weakened by staurosporine. The Ca2+ channel blocker - verapamil partially inhibited the effects of LLLI. Thus, lipid peroxidation was reduced and ATP concentration and sperm motility were increased during cryopreservation under LLLI. These properties of LLLI are associated with cell signaling pathways.
| Attachment | Size |
|---|---|
 09_109-113.pdf | 756.62 KB |
