Investigation of Long Non-Coding RNA Gene Polymorphisms LINC02952, LINC02747, LINC02664, MALAT-1 AND H19 in Clear Cell Renal Cell Carcinoma Patients From the Volga-Ural Region

Clear cell renal cell carcinoma (ccRCC) is a highly aggressive form of cancer that frequently recurs and metastasizes, necessitating the search for novel molecular markers to enhance diagnosis and prognosis. Single nucleotide polymorphisms (SNPs) in long non-coding RNA (lncRNA) genes, which have a pivotal role in tumorigenesis, represent promising candidates for such biomarkers. The TaqMan allele discrimination method of genotyping of six SNPs (rs11263432, rs4506680, rs793096, rs619586, rs3200401, and rs3741219) within lncRNA genes (LINC02952, LINC02747, LINC02664, MALAT-1 and H19) was performed on 128 patients with ccRCC from the Tatar population and 134 healthy control individuals, who were comparable in terms of gender, age, and region of residence. The statistical analysis assessed the association between genotypes and the risk of ccRCC development. Significant associations with the risk of developing ccRCC have been identified. The rs11263432*T, rs79396*T, and rs619586*A alleles were associated with an increased risk of the disease (OR = 2.32 (95%CI = 1.1-5.3) p = 0.04, OR = 1.49 (95%CI = 1.0-2.1) p = 0.03 and OR = 1.33 (95%CI = 0.3-6.7) p = 0.03, respectively). No statistically significant associations with ccRCC risk were found for rs4506680, rs3200401, and rs3741219 polymorphisms. The results of the study indicate that specific polymorphisms in the lncRNA LINC02952, LINC02664 and MALAT-1 genes may serve as potential markers of predisposition to ccRCC in the studied population. The findings highlight the important role of variations in lncRNA genes in ccRCC pathogenesis and their possible potential as targets for developing new approaches to personalized diagnosis and risk assessment.