Redox dynamics in mononuclear cells during phagocytosis and post-exposure to UV radiation was evaluated in vitro. Mononuclear cells were isolated from rat blood using density gradient centrifugation. Phagocytosis was activated via latex beads, and redox processes were monitored at 1, 30, and 60 minutes in control samples and after UV exposure at doses of 1, 5, and 15 J/m2. Cell viability post-phagocytosis was assessed using propidium iodide staining and cytofluorimetry. Redox processes in phagocytes were evaluated by measuring fluorescence of tryptophan, Schiff bases and glycated proteins, alongside absorption spectra/florescence levels of metabolic coenzymes FAD, NAD(P) and NAD(P)H and their ratios. In controls, cell viability decreased by 15.2% after 60 minutes of phagocytosis, while tryptophan, NAD(P), and NAD(P)H levels remained stable. Concentrations of Schiff bases and glycated proteins doubled. UV radiation significantly reduced cell viability in a dose-dependent manner. At 15 J/m2, 80.99% of cells were nonviable after 60 minutes of phagocytosis. This dose also caused a 21.42-fold decrease in tryptophan fluorescence, a 3.8-fold reduction in Schiff bases/ glycated proteins, and declined metabolic coenzyme levels. Ratios of NAD(P)H/FAD and NAD(P)H/NAD(P) decreased post-UV exposure, indicating oxidative dominance and NAD(P)H deficiency. However, despite UV-induced cytotoxicity, all experimental series, regardless of the dose of UV discharge, maintained control trends: non-enzymatic glycation products (Schiff bases, glycated proteins) increased, and the NAD(P)H/FAD ratio rose due to reduced coenzyme accumulation during phagocytosis. This suggests that phagocytic redox pathways persist under UV stress, albeit at diminished intensity.
| Attachment | Size |
|---|---|
 02_13-26.pdf | 1.12 MB |
