Opera Medica et Physiologica

Cultivation of Primary Hippocampal Cell Cultures for the Functional and Morphological Matching Organization of Single Neurons

Abstract: 

Correlation microscopy is an invaluable tool for studying the functional and structural features of individual cells. Here we present an approach for the cultivation of primary hippocampal cultures grown on a special substrate that allows drawing a convenient coordinate grid. The metabolic activity and immunocytochemical labeling of neurons were estimated, as well as the patch-clamp method and ultrastructural analysis.