The State of Nitrogen Metabolism Under the Influence of Exogenous NO in Experimental Thermal Trauma

The effect of therapeutic technologies used in the treatment of burns on the parameters of nitrogen metabolism has not been sufficiently studied. In this regard, the assessment of the level of nitrites and nitrates of blood and tissues in combined thermal trauma (CTT) under the influence of NO is of scientific and practical interest. The aim of the study was to determine the concentration of nitric oxide metabolites in the blood and organs under the influence of NO during CTT. The experiment was carried out on white male rats of the Wistar line. CTT (contact burn on the area of 20% of the body surface and thermal inhalation exposure for 20-30 seconds) was applied under anesthesia. Animals with CTT were treated daily with intraperitoneal injections of a 10% dinitrosyl iron complexes (DNIC) solution or were inhaled with NO (20ppm). The concentration of nitrites and nitrates was determined in the blood plasma and homogenate of the liver, heart, kidneys, and lungs. CTT was accompanied by hyperproduction of NO in blood plasma on the 1st, 7th and 10th days after injury with a maximum growth on the 1st day. The largest decrease in NO_2- and NO_3- levels during CTT was observed in the kidneys > liver > lungs > heart. The use of DNIC in CTT led to an increase in NO_2- and NO_3- in the organs. Inhalations of NO during CTT increased the concentration of NO_2- and NO_3-  in the homogenate of the lungs > kidneys > heart > liver.