Opera Medica et Physiologica

Possible Applications of Scanning Electron Microscopy in Structure and Composition Analysis of Intra-Articular Distracted Formations of Proxi-mal Interphalangeal Joints in Humans

Published ahead of print June 04, 2023; Printed June 05, 2023; OM&P 2023 Volume 10 Issue 2, pages 41-50; doi:10.24412/2500-2295-2023-2-41-50

This research evaluates particularities of surface structural organization of surgical samples of intracapsular distracted formations in treating post-traumatic fibrous ankylosis of proximal interphalangeal joints by means of scanning electron microscopy. The research is carried out on small-fragment samples of intracapsular formations collected in the course of treatment from 8 patients of the Microsurgical Department. Morphological evaluation of the surface of two types of samples was performed: that of proximal interphalangeal joint capsules, and of the regenerate itself. Structural analysis of the surgical samples allowed to identify considerable differences both in form and size of single structure-forming elements, and in their organizational types. It was established that the regenerate has a more compact structure. In the course of microanalysis of elemental composition, it was ascertained that mass percentage of calcium and phosphorus in the examined regenerate samples is 4.4 and 2.2 times respectively higher compared to the capsule samples. These results could be used in further research into approaches to the evaluation of connective tissue regeneration after surgeries, as well as in making recommendations for operative or physiotherapeutic patient treatment in cases of joint capsule distraction.