The present study investigated the effects of novel Xymedon conjugates with para-aminobenzoic acid (PABA) and succinic acid on the regeneration processes of the model organism, the planarian Schmidtea mediterranea Benazzi, Baguñà, Ballester, Puccinelli & Del Papa, 1975 (Platyhelminthes, Tricladida). Blastema regeneration and cellular proliferative activity were quantitatively assessed using vital computer morphometry and flow cytofluorometry. The results showed that the Xymedon conjugate with PABA at a concentration of 5 mg/100 mL significantly increased the regeneration index and stimulated cell proliferation by elevating the proportion of cells in the S and G₂/M phases of the cell cycle. In contrast, the Xymedon conjugate with succinic acid showed no stimulatory effect. At the highest tested concentration (0.01 mg/100 mL), it slightly suppressed regeneration and reduced the fraction of cells in the G₂/M phase. These results confirm S. mediterranea feasibility as a model for screening the regenerative potential of biologically active compounds and highlight Xymedon's significance as a structural platform for developing new regeneration stimulators.
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