The Effect of 1,2,4-trioxolanes with Betulin in Fish Oil on Oxidative and Energy Metabolism Under Hypoxia and Immobilization Stress in Rats

1,2,4-trioxolanes were obtained by the ozonation of fish oil with a mixture of oxygen and ozone to study their physicochemical properties. The biological activity of 1,2,4-trioxolanes with betulin from birch bark extract in fish oil was evaluated under hypoxia and immobilization stress in rats. 1,2,4-trioxolanes composition led to LPO indexes normalization (malondialdehyde, Schiff bases, diene and triene conjugates), the activation of NADP/H and NAD/H-dependent enzymes (GR, G6PDH, LDH, AlDH), as well as SOD and catalase, under stress in rats. Thus, we estimated 1,2,4-trioxolanes with betulin in fish oil to regulate oxidative and energy metabolism under hypoxia and immobilization stress in rats. The findings show that studied composition can be useful to prevent and treat the diseases caused by oxidative stress.