Opera Medica et Physiologica

Amphotericin B as a Cholesterol Identifier in Human Erythrocyte's Membrane

Published ahead of print March 20, 2022; Printed March 21, 2022; OM&P 2022 Volume 9 Issue 1, pages 42-48; doi:10.24412/2500-2295-2022-1-42-48
Abstract: 

Conditions were determined for the identification of cholesterol in erythrocyte membranes by the fluorescent method using amphotericin B as a probe. We performed a comparative analysis of electronic absorption and fluorescence spectra of cholesterol, amphotericin B, and the products of their interaction, and determined the optimal wavelengths for excitation (328 nm) and recording (468 nm) of fluorescence. Amphotericin B stained cholesterol in membranes was visualized by fluorescence microscopy. It was shown that the registration of emission band at 468 nm on fluorescence spectra of free and membrane-bound cholesterol can serve as a marker of the presence of this lipid in the samples.