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The effect of therapeutic technologies used in the treatment of burns on the parameters of nitrogen metabolism has not been sufficiently studied. In this regard, the assessment of the level of nitrites and nitrates of blood and tissues in combined thermal trauma (CTT) under the influence of NO is of scientific and practical interest. The aim of the study was to determine the concentration of nitric oxide metabolites in the blood and organs under the influence of NO during CTT. The experiment was carried out on white male rats of the Wistar line. CTT (contact burn on the area of 20% of the body surface and thermal inhalation exposure for 20-30 seconds) was applied under anesthesia. Animals with CTT were treated daily with intraperitoneal injections of a 10% dinitrosyl iron complexes (DNIC) solution or were inhaled with NO (20ppm). The concentration of nitrites and nitrates was determined in the blood plasma and homogenate of the liver, heart, kidneys, and lungs. CTT was accompanied by hyperproduction of NO in blood plasma on the 1st, 7th and 10th days after injury with a maximum growth on the 1st day. The largest decrease in NO_2- and NO_3- levels during CTT was observed in the kidneys > liver > lungs > heart. The use of DNIC in CTT led to an increase in NO_2- and NO_3- in the organs. Inhalations of NO during CTT increased the concentration of NO_2- and NO_3-  in the homogenate of the lungs > kidneys > heart > liver.

Physico-chemical characteristics of optical-induced break-down depend weakly on the protein concentration. This factor distinguishes lysozyme from other aqueous solutions which contain objects with nano-size. The laser radiation of the lysozyme aqueous solution during different time periods shows patterns that are quite similar to the classical patterns. In this particular paper, it has been observed that the catalytic activity of lysozyme is decreasing after the laser radiation. The optical density of the protein solutions is increasing. The fluorescence intensity of the lysozyme solution is determined by the amino-acid residues. It decreases before and after the laser light influence. The position of the maximum of the excitation and emission did not change. The shape of the fluorescence signal on the 3D maps also does not change significantly. This observation suggests the degradation of the amino-acid residues. The viscosity and the pseudo plasticity of the lysozyme aqueous solutions increased. However, there is no massive damage of the polypeptide chain. On the contrary, the optical break-down shows the intensive forming of the lysozyme aggregates.

The high risk of ovarian cancer is primarily associated with mutations in BRCA1 and BRCA2 genes. However, mutations in these explain only a small proportion of cases. Mutations in other genes are also involved in the disease. As a result of previous exome sequencing of DNA samples from breast cancer Germany patients with clinical signs of a hereditary form of the disease without major mutations in the BRCA1, BRCA2, CHEK2 and NBN genes, potentially pathogenic genetic variants in new breast and ovarian cancer candidate genes were selected. Selected as a result of bioinformatics analysis genes are involved in vital cell signaling pathways such as repair, apoptosis, cell cycle regulation, cell proliferation, migration, differentiation, as well as immune response and inflammation. Recently, biological microarray technologies have been widely used to study the general genetic variability throughout the human genome in order to determine genetic associations with the disease and search for genes involved in the pathogenesis of multifactorial pathologies. The use of such approaches can be very useful for identifying risk markers for the development and severity of diseases. Our case-control study is aimed at researching potentially pathogenic variants selected as a result of exome sequencing of DNA samples from Caucasian patients using microarray technology Fluidigm to assess their contribution to ovarian cancer pathogenesis in Bashkortostan. Most of the researched alleles were found with different frequencies among cases and controls; however, our data indicate that the researched potentially pathogenic variants do not contribute to ovarian cancer pathogenesis in Bashkortostan populations.

The article considers the development of biocomposite materials as bacterial cellulose-based hydrogel, chitosan and alginate, plus physiologically active compounds – fusidic acid, resveratrol and dihydroquercetin. It has been found that the use of hydrogel systems derived from microbial polysaccharides and containing bacterial cellulose (BC) / sodium alginate with CaCl₂ /sodium fusidate (SF) and BC/chitosan /sodium fusidate helps to reduce the intensity of lipid peroxidation processes and stabilize phospholipid and fatty acid composition of the skin. It is consistent with the findings of the authors on longer release of sodium fusidate from biocomposite materials of this composition. Therefore, the use of BC and chitosan in combination with sodium fusidate, which exhibits antibacterial properties, and the crosslinking of sodium alginate with calcium chloride solution proves to be the most effective for restoring the skin’s lipid composition and shortening the course of treatment. Most likely, this effect must be explained by the constant release of physiologically active compounds from hydrogel composites and its impact on damaged skin areas.

The visceral system can influence consciousness and emotions. In this paper, we investigated whether the processing of short-term emotional stimuli along different phases of the cardiac cycle is selectively regulated. Emotional and neutral faces were presented to the volunteers during cardiac systole, when the release of blood from the heart causes arterial baroreceptors to centrally signal the strength and time of the heartbeat, and in diastole, the period between heartbeats when the baroreceptors are at rest. Participants passively observed the faces appearing in the oddball paradigm in immersive virtual reality (VR). Combining electroencephalography and electrocardiography, cardio synchronous ERP was recorded. The ERP data at the occipital Oz electrode demonstrate the dependence of emotional stimuli in respect to the cardiac cycle. ERP in VR were higher during diastole than systole. More specifically, the cardiac phase influenced late component P300 of the ERP, did not influence early P100 and affected P200 only to angry face. This new evidence that events related to cardiac function can modulate emotional perception in virtual reality might be one more prime example of how body feedback shapes emotions.

The paper focuses on studying the rheological properties of erythrocytes in patients with a new coronavirus infection in the acute period and during early convalescence. A significant decrease in erythrocyte membrane plasticity was found in all patients in the acute period of the disease and every second recovering patients in the early post-COVID period. An increase in the degree and rate of erythrocyte aggregation in a quarter of cases, regardless of the observation period, was accompanied by inhibition of erythrocyte disaggregation mechanisms, which can serve as a pathophysiological basis for the formation of hemorheological disorders both in the micro- and macrovasculature in the first 3 months after recovery.